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Optimization and evaluation of single-cell whole-genome multiple displacement amplification

1 Jan 2006

Abstract excerpt

The scarcity of genomic DNA can be a limiting factor in some fields of genetic research. One of the methods developed to overcome this difficulty is whole genome amplification (WGA). Recently, multiple displacement amplification (MDA) has proved very efficient in the WGA of small DNA samples and pools of cells, the reaction being catalyzed by the phi29 or the Bst DNA polymerases. The aim of the present study was...

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PMID: 16619243
DOI: 10.1002/humu.20324

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