Article
Optimization of chondrocyte isolation and phenotype characterization for cartilage tissue engineering.
Tissue engineering. Part C, Methods - 1 Feb 2015
Lau Ting Ting, Peck Yvonne, Huang Weiliang, Wang Dong-An
Abstract excerpt
Current protocols for chondrocyte isolation are inconsistent, resulting in suboptimal cell yield and compromised cell quality. Thus, there is a need for an improved isolation protocol that is able to give a maximum yield with optimal cell viability while preserving the chondrocyte phenotype. In light of this, we developed an improved isolation protocol based on enzymatic digestion using 0.1% (w/v) collagenase II....
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