Article
Regional effects of enzymatic digestion on knee meniscus cell yield and phenotype for tissue engineering.
Tissue engineering. Part C, Methods - 1 Mar 2012
Sanchez-Adams Johannah, Athanasiou Kyriacos A
Abstract excerpt
An abundant cell source is the cornerstone of most tissue engineering strategies, but extracting cells from the knee meniscus is hindered by its dense fibrocartilaginous matrix. Identifying a method to efficiently isolate meniscus cells is important, as it can reduce the cost and effort required to perform meniscus engineering research. In this study, six enzymatic digestion regimens used for cartilaginous cell...
Topics
- Animals
- Cattle
- Cell Count
- Cell Separation
- Cells, Cultured
- Collagenases
- Efficiency
- Knee Joint
- Menisci, Tibial
- Necrosis
- Phenotype
- Proteolysis
- Tissue Engineering