Article
Immortalization of human cells by mutant and chimeric primate polyomavirus T-antigen genes.
Oncogene - 16 Mar 1995
O'Neill F J, Frisque R J, Xu X, Hu Y X, Carney H
Abstract excerpt
Human fibroblasts were morphologically transformed with wild type and mutant SV40 T-antigens (T-Ags) and with SV40/JCV and SV40/BKV chimeric T-Ags. The transformants were then assayed for the attainment of immortal cell growth. Several observations relating T-Ag and T-Ag domains to immortalization were made. Approximately 10% of SV40-transformants became immortal. Transformants generated by transfection or...
Topics
- Antigens, Polyomavirus Transforming
- Blotting, Southern
- Cell Transformation, Viral
- Cells, Cultured
- DNA, Viral
- Fibroblasts
- Humans
- Mutation
- Recombinant Fusion Proteins
- Tumor Suppressor Protein p53