Mutations at nucleotides 573 and 579 within 5'-untranslated region augment the virulence of coxsackievirus B1.

doi:10.1016/j.virusres.2008.04.012

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Article

Mutations at nucleotides 573 and 579 within 5'-untranslated region augment the virulence of coxsackievirus B1.

Virus research - 1 Aug 2008

Zhong Zhaohua, Li Xiaobo, Zhao Wenran, Tong Lei, Liu Jian, Wu Shuaiqin, Lin Lexun, Zhang Zhonghai, Tian Ye, Zhang Fengmin

Abstract excerpt

Two mutants of coxsackievirus B1 (CVB1), CVB1c and CVB1e, with mutations in the stem-loop H of 5'-UTR were generated by site-directed mutagenesis. The A at nt579 of CVB1c was substituted by G. The U at nt573 and A at nt579 of CVB1e were substituted by A and G. The virulences of these mutants had been assessed by means of cytopathic effect (CPE), plaque formation, one-step growth curve, and 50% lethal dose (LD50)...

Topics

5' Untranslated Regions
Animals
Animals, Suckling
Base Sequence
Enterovirus B, Human
HeLa Cells
Humans
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation
Virulence

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PMID: 18514346
DOI: 10.1016/j.virusres.2008.04.012

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