Article
Development of a reversibly switchable fluorescent protein for super-resolution optical fluctuation imaging (SOFI).
ACS nano - 24 Mar 2015
Zhang Xi, Chen Xuanze, Zeng Zhiping, Zhang Mingshu, Sun Yujie, Xi Peng, Peng Jianxin, Xu Pingyong
Abstract excerpt
Reversibly switchable fluorescent proteins (RSFPs) can be effectively used for super-resolution optical fluctuation imaging (SOFI) based on the switching and fluctuation of single molecules. Several properties of RSFPs strongly influence the quality of SOFI images. These properties include (i) the averaged fluorescence intensity in the fluctuation state, (ii) the on/off contrast ratio, (iii) the photostability,...
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