Detection of T-cell receptor gamma chain V gene rearrangements using the polymerase chain reaction: application to the study of clonal disease cells in acute lymphoblastic leukemia.

Abstract excerpt

This report describes the development and characterization of a method for the amplification of rearranged V-J segments of the human T-cell receptor gamma chain (TCRG) locus using an adaptation of the polymerase chain reaction (PCR) technique. The technique uses a single pair of 'consensus' primers to amplify rearrangements involving the V gamma I subgroup genes, which are common in malignant cells from acute...

Topics

• Alleles
• Base Sequence
• Clone Cells
• DNA, Neoplasm
• Deoxyribonuclease EcoRI
• Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor
• Humans
• Leukemia-Lymphoma, Adult T-Cell
• Molecular Sequence Data
• Polymerase Chain Reaction