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Article

A high-throughput screen for hyaluronic acid accumulation in recombinant Escherichia coli transformed by libraries of engineered sigma factors.

Biotechnology and bioengineering - 1 Nov 2008

Yu Huimin, Tyo Keith, Alper Hal, Klein-Marcuschamer Daniel, Stephanopoulos Gregory

Abstract excerpt

Hyaluronic acid (HA) is an important biomaterial with functional medical and cosmetic applications. As its synthesis has been recently reported in recombinant bacteria, it is of interest to develop a high throughput screening method for the rapid isolation of HA accumulating strains transformed by combinatorial libraries. Here we report a novel two-step screening strategy to select for better HA-producing...

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PMID: 18500764
DOI: 10.1002/bit.21947

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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