Consensus mutagenesis reveals that non-helical regions influence thermal stability of horseradish peroxidase.

Abstract excerpt

The enzyme horseradish peroxidase has many uses in biotechnology but a stabilized derivative would have even wider applicability. To enhance thermal stability, we applied consensus mutagenesis (used successfully with other proteins) to recombinant horseradish peroxidase and generated five single-site mutants. Unexpectedly, these mutations had greater effects on steady-state kinetics than on thermal stability....

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Enzyme Stability
• Share to Horseradish Peroxidase
• Share to Kinetics