Article
From DNA sequence to improved functionality: using protein sequence comparisons to rapidly design a thermostable consensus phytase.
Protein engineering - 1 Jan 2000
Lehmann M, Kostrewa D, Wyss M, Brugger R, D'Arcy A, Pasamontes L, van Loon A P
Abstract excerpt
Naturally-occurring phytases having the required level of thermostability for application in animal feeding have not been found in nature thus far. We decided to de novo construct consensus phytases using primary protein sequence comparisons. A consensus enzyme based on 13 fungal phytase sequences had normal catalytic properties, but showed an unexpected 15-22 degrees C increase in unfolding temperature compared...
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