Article
A range of catalytic efficiencies with avian retroviral protease subunits genetically linked to form single polypeptide chains.
The Journal of biological chemistry - 15 Mar 1991
Bizub D, Weber I T, Cameron C E, Leis J P, Skalka A M
Abstract excerpt
Molecular modeling based on the crystal structure of the Rous sarcoma virus (RSV) protease dimer has been used to link the two identical subunits of this enzyme into a functional, single polypeptide chain resembling the nonviral aspartic proteases. Six different linkages were selected to test the...
Topics
- Avian Sarcoma Viruses
- Base Sequence
- Blotting, Western
- Catalysis
- Electrophoresis, Polyacrylamide Gel
- Endopeptidases
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Gene Expression Regulation, Enzymologic
- Inclusion Bodies
- Kinetics
- Molecular Sequence Data
- Mutation
- Plasmids
- Protein Conformation