Article
Vector construction for gene overexpression as a tool to elucidate gene function.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2003
Lloyd Alan
Abstract excerpt
Gene overexpression as a means to determine plant gene function has been used almost since the first plant transformation protocols became viable. The goal of these experiments, as in classical genetic experiments, is to observe any phenotypic change associated with changing the expression of a gene of interest-in this case overexpression. Any phenotypic changes are interpreted, and the native gene's function is...
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