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Article

Mutants of human insulin-like growth factor II with altered affinities for the type 1 and type 2 insulin-like growth factor receptor.

The Journal of biological chemistry - 15 Jan 1991

Bürgisser D M, Roth B V, Giger R, Lüthi C, Weigl S, Zarn J, Humbel R E

Abstract excerpt

With the aim to produce insulin-like growth factors (IGF) with enhanced specificity for the type 1 or type 2 IGF receptors, three mutants of IGF II have been prepared and expressed in NIH-3T3 cells. IGF II mutated at Tyr27 to Leu and Glu showed a 25- and 54-fold decrease in affinity for the type 1 IGF receptor and a 3.4- and 9.2-fold decrease in affinity for the type 2 IGF receptor. IGF II mutated at Phe48 to Glu...

Topics

Amino Acid Sequence
Base Sequence
Blotting, Western
Cell Line
Cross-Linking Reagents
Electrophoresis, Polyacrylamide Gel
Humans
Insulin-Like Growth Factor II
Molecular Sequence Data
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Radioimmunoassay

Radioligand Assay

Receptors, Cell Surface

Receptors, Somatomedin

Thymidine

Source

Identifiers

PMID: 1845984

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1845984-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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