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Article

Simultaneous detection of multiple point mutations using allele-specific oligonucleotides.

Current protocols in human genetics - 1 Sept 2004

Napolitano Nichole M, Rohlfs Elizabeth M, Heim Ruth A

Abstract excerpt

This unit describes high-throughput mutation analysis using hybridization with pooled allele-specific oligonucleotide (ASO) probes. The approach can be used to screen one gene for many allelic mutations or to screen several loci for several allelic mutations each. Because tetramethyl ammonium chloride (TMAC) is added to the hybridization solution, the melting temperature of each oligonucleotide is independent of...

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PMID: 18428368
DOI: 10.1002/0471142905.hg0904s41

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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