Article
Unravelling genetic data by arrayed primer extension.
Clinical chemistry and laboratory medicine - 1 Feb 2000
Tõnisson N, Kurg A, Kaasik K, Lõhmussaar E, Metspalu A
Abstract excerpt
We have developed a method for arrayed primer extension (APEX) on an oligonucleotide microchip together with the 4-color fluoresence imaging equipment and supporting software, that allows analysis of the DNA sequence and changes in it. Mutation analysis of BRCA1 gene and single nucleotide polymorphism (SNP) chip for genotyping were used as a model system. Chip surface chemistry, template preparation and APEX...
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