Article
Identification of a physiological phosphorylation site of the herpes simplex virus 1-encoded protein kinase Us3 which regulates its optimal catalytic activity in vitro and influences its function in infected cells.
Journal of virology - 1 Jul 2008
Kato Akihisa, Tanaka Michiko, Yamamoto Mayuko, Asai Risa, Sata Tetsutaro, Nishiyama Yukihiro, Kawaguchi Yasushi
Abstract excerpt
Us3 is a serine/threonine protein kinase encoded by herpes simplex virus 1 (HSV-1). Here, we report the identification of a physiological Us3 phosphorylation site on serine at position 147 (Ser-147) which regulates its protein kinase activity in vitro. Moreover, mutation of this site influences Us3 function, including correct localization of the enzyme and induction of the usual morphological changes in...
Topics
- Animals
- Catalysis
- Catalytic Domain
- Chlorocebus aethiops
- Cyclic AMP-Dependent Protein Kinases
- DNA Primers
- Escherichia coli
- Fluorescent Antibody Technique
- Genome, Viral
- Immunoblotting
- Immunoprecipitation
- Mutagenesis