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Article

Development and validation of a cell-based fluorescent method for measuring antibody affinity.

Journal of immunological methods - 1 Mar 2017

Yu Xin, Pegram Charles N, Bigner Darell D, Chandramohan Vidyalakshmi

Abstract excerpt

Monoclonal antibodies have become essential tools for diagnostic and therapeutic purposes. Antibody affinity is one of the critical factors influencing the therapeutic success of tumor-targeting antibodies. Therefore, developing an accurate and reliable method for determining antibody affinity is crucial. In this study, we describe a fluorescent cell-based immunosorbent assay that can accurately measure antibody...

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Share to Antibodies, Monoclonal
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Binding, Competitive

Share to Binding Sites, Antibody

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Share to Chondroitin Sulfate Proteoglycans

Share to Spectrometry, Fluorescence

Source

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Identifiers

PMID: 28024998
DOI: 10.1016/j.jim.2016.12.004
PMCID: PMC5293658

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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