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Article

Structural determinants of glutathione transferases with azathioprine activity identified by DNA shuffling of alpha class members.

Journal of molecular biology - 1 Feb 2008

Kurtovic Sanela, Modén Olof, Shokeer Abeer, Mannervik Bengt

Abstract excerpt

A library of alpha class glutathione transferases (GSTs), composed of chimeric enzymes derived from human (A1-1, A2-2 and A3-3), bovine (A1-1) and rat (A2-2 and A3-3) cDNA sequences was constructed by the method of DNA shuffling. The GST variants were screened in bacterial lysates for activity with the immunosuppressive agent azathioprine, a prodrug that is transformed into its active form, 6-mercaptopurine, by...

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Identifiers

PMID: 18155239
DOI: 10.1016/j.jmb.2007.11.034

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Structural determinants of glutathione transferases with azathioprine activity identified by DNA shuffling of alpha class members.PMID 18155239Read ↗

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