Article
Optimized and far-red-emitting variants of fluorescent protein eqFP611.
Chemistry & biology - 1 Mar 2008
Kredel Simone, Nienhaus Karin, Oswald Franz, Wolff Michael, Ivanchenko Sergey, Cymer Florian, Jeromin Andreas, Michel Francois J, Spindler Klaus-Dieter, Heilker Ralf, Nienhaus G Ulrich, Wiedenmann Jörg
Abstract excerpt
Fluorescent proteins (FPs) emitting in the far-red region of the spectrum are highly advantageous for whole-body imaging applications because scattering and absorption of long-wavelength light is markedly reduced in tissue. We characterized variants of the red fluorescent protein eqFP611 with bright fluorescence emission shifted up to 639 nm. The additional red shift is caused by a trans-cis isomerization of the...
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