Article
Random mutagenesis and recombination of sam1 gene by integrating error-prone PCR with staggered extension process.
Biotechnology letters - 1 Jul 2008
An Yingfeng, Ji Jianfei, Wu Wenfang, Huang Ribo, Wei Yutuo, Xiu Zhilong
Abstract excerpt
An efficient method for creating a DNA library is presented in which gene mutagenesis and recombination can be introduced by integrating error-prone PCR with a staggered extension process in one test tube. In this process, less than 15 cycles of error-prone PCR are used to introduce random mutati...
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