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A sensitive mass spectrum assay to characterize engineered methionine adenosyltransferases with S-alkyl methionine analogues as substrates.

Analytical biochemistry - 1 Apr 2014

Wang Rui, Zheng Weihong, Luo Minkui

Abstract excerpt

Methionine adenosyltransferases (MATs) catalyze the formation of S-adenosyl-l-methionine (SAM) inside living cells. Recently, S-alkyl analogues of SAM have been documented as cofactor surrogates to label novel targets of methyltransferases. However, these chemically synthesized SAM analogues are not suitable for cell-based studies because of their poor membrane permeability. This issue was recently addressed...

Topics

Amino Acid Sequence
Animals
Chromatography, High Pressure Liquid
Enzyme Assays
Humans
Kinetics
Methionine Adenosyltransferase
Molecular Sequence Data
Mutation
Protein Engineering
Rats

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

S-Adenosylmethionine

Substrate Specificity

Tandem Mass Spectrometry

Source

Open in PubMed Open full text

Identifiers

PMID: 24374249
DOI: 10.1016/j.ab.2013.12.026
PMCID: PMC3947680

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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