Mutations on the N-terminal region abolish differentially the enzymatic activity, membrane-damaging activity and cytotoxicity of Taiwan cobra phospholipase A2.

Abstract excerpt

To examine the functional contribution of the N-terminal region to the activities of Naja naja atra phospholipase A(2) (PLA(2)), studies on three N-terminally mutated PLA(2) were carried out in the present work. Removal of N-terminal heptapeptide caused a complete loss of membrane-damaging activity, whilst the mutants with an extra Met before Asn-1 or substituting Asn-1 with Met still retained approximately 40.9%...

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