Article
Simultaneous isolation of DNA and RNA from the same cell population obtained by laser capture microdissection for genome and transcriptome profiling.
The Journal of molecular diagnostics : JMD - 1 Mar 2008
Xu Chang, Houck John R, Fan Wenhong, Wang Pei, Chen Yu, Upton Melissa, Futran Neal D, Schwartz Stephen M, Zhao Lue P, Chen Chu, Mendez Eduardo
Abstract excerpt
Laser capture microdissection (LCM) is used extensively for genome and transcriptome profiling. Traditionally, however, DNA and RNA are purified from separate populations of LCM-harvested cells, limiting the strength of inferences about the relationship between gene expression and gene sequence variation. There have been no published protocols for the simultaneous isolation of DNA and RNA from the same cells that...
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