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Article

Separation and parallel sequencing of the genomes and transcriptomes of single cells using G&T-seq.

Nature protocols - 1 Nov 2016

Macaulay Iain C, Teng Mabel J, Haerty Wilfried, Kumar Parveen, Ponting Chris P, Voet Thierry

Abstract excerpt

Parallel sequencing of a single cell's genome and transcriptome provides a powerful tool for dissecting genetic variation and its relationship with gene expression. Here we present a detailed protocol for G&T-seq, a method for separation and parallel sequencing of genomic DNA and full-length polyA(+) mRNA from single cells. We provide step-by-step instructions for the isolation and lysis of single cells; the...

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PMID: 27685099
DOI: 10.1038/nprot.2016.138

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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