Article

A modified dual preparatory method for improved isolation of nucleic acids from laser microdissected fresh-frozen human cancer tissue specimens

2024-08-02

Abstract excerpt

A central theme in cancer research is to increase our understanding of the cancer tissue microenvironment (TME), which is comprised of a complex and spatially heterogeneous ecosystem of malignant and non-malignant cells, both of which actively contribute to an intervening extracellular matrix. Laser microdissection (LMD) enables histology selective harvest of cellular subpopulations from the tissue microenvironmen...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 156c311e-9060-5c7f-b9f5-ef200685adbc
DOI: 10.1101/2024.08.02.606193

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A modified dual preparatory method for improved isolation of nucleic acids from laser microdissected fresh-frozen human cancer tissue specimensDOI 10.1101/2024.08.02.606193Read

Simultaneous isolation of DNA and RNA from the same cell population obtained by laser capture microdissection for genome and transcriptome profiling.Semantic proximity Read

Optimizing nucleic acid extraction and transcriptome evaluation from low-input, fixed clinical samplesSemantic proximity Read

Standardization of the preanalytical phase of DNA extraction from fixed tissue for next-generation sequencing analysesSemantic proximity Read

Efficiency of EGFR mutation analysis for small microdissected cytological specimens using multitech DNA extraction solution.Semantic proximity Read

Optimizing Workflows and Processing of Cytologic Samples for Comprehensive Analysis by Next-Generation Sequencing: Memorial Sloan Kettering Cancer Center ExperienceSemantic proximity Read

A simple, precise and economical microdissection technique for analysis of genomic DNA from archival tissue sectionsSemantic proximity Read

DNA Quantity and Quality Comparisons between Cryopreserved and FFPE Tumors from Matched Pan-Cancer SamplesSemantic proximity Read

Reliable RNA-seq analysis from FFPE specimens as a means to accelerate cancer-related health disparities researchSemantic proximity Read

High-quality RNA and DNA from flow cytometrically sorted human epithelial cells and tissues.Semantic proximity Read

An automated and fast sample preparation workflow for laser microdissection guided ultrasensitive proteomicsSemantic proximity Read

A cost-effective and scalable approach for DNA extraction from FFPE tissuesSemantic proximity Read

Cell-type-resolved spatial proteogenomics from matched genome and proteome of the same cellsSemantic proximity Read