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Article

Rapid quantification of global DNA methylation by isocratic cation exchange high-performance liquid chromatography.

Analytical biochemistry - 15 Apr 2008

Rozhon Wilfried, Baubec Tuncay, Mayerhofer Juliane, Mittelsten Scheid Ortrun, Jonak Claudia

Abstract excerpt

The DNA of many eukaryotes is methylated at specific cytosine residues in connection with gene regulation. Here we report a method for the quantification of global cytosine methylation based on enzymatic hydrolysis of DNA, dephosphorylation, and subsequent high-performance cation exchange chromatography. Nucleosides are separated in less than 3 min under isocratic conditions on a benzenesulfonic acid-modified...

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PMID: 18249178
DOI: 10.1016/j.ab.2008.01.001

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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