Article
Subnuclear partitioning of rRNA genes between the nucleolus and nucleoplasm reflects alternative epiallelic states.
Genes & development - 15 Jul 2013
Pontvianne Frederic, Blevins Todd, Chandrasekhara Chinmayi, Mozgová Iva, Hassel Christiane, Pontes Olga M F, Tucker Sarah, Mokros Petr, Muchová Veronika, Fajkus Jirí, Pikaard Craig S
Abstract excerpt
Eukaryotes can have thousands of 45S ribosomal RNA (rRNA) genes, many of which are silenced during development. Using fluorescence-activated sorting techniques, we show that active rRNA genes in Arabidopsis thaliana are present within sorted nucleoli, whereas silenced rRNA genes are excluded. DNA methyltransferase (met1), histone deacetylase (hda6), or chromatin assembly (caf1) mutants that disrupt silencing...
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