Article
Quality scores and SNP detection in sequencing-by-synthesis systems.
Genome research - 1 May 2008
Brockman William, Alvarez Pablo, Young Sarah, Garber Manuel, Giannoukos Georgia, Lee William L, Russ Carsten, Lander Eric S, Nusbaum Chad, Jaffe David B
Abstract excerpt
Promising new sequencing technologies, based on sequencing-by-synthesis (SBS), are starting to deliver large amounts of DNA sequence at very low cost. Polymorphism detection is a key application. We describe general methods for improved quality scores and accurate automated polymorphism detection, and apply them to data from the Roche (454) Genome Sequencer 20. We assess our methods using known-truth data sets,...
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