Article
PAP-LMPCR for improved, allele-specific footprinting and automated chromatin fine structure analysis.
Nucleic acids research - 1 Feb 2008
Ingram R, Gao C, Lebon J, Liu Q, Mayoral R J, Sommer S S, Hoogenkamp M, Riggs A D, Bonifer C
Abstract excerpt
The analysis of chromatin fine structure and transcription factor occupancy of differentially expressed genes by in vivo footprinting and ligation-mediated-PCR (LMPCR) is a powerful tool to understand the impact of chromatin on gene expression. However, as with all PCR-based techniques, the accuracy of the experiments has often been reduced by sequence similarities and the presence of GC-rich or repeat sequences,...
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