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Article

Pyrophosphorolysis-activated polymerization (PAP): application to allele-specific amplification.

BioTechniques - 1 Nov 2000

Liu Q, Sommer S S

Abstract excerpt

To measure mutation load or to detect minimal residual disease, a robust method for identifying one mutant allele in the range of 10(6)-10(9) wild-type alleles would be advantageous. Herein, we present evidence that pyrophosphorolysis-activated polymerization (PAP) has the potential to provide a highly specific and robust method of allele-specific amplification if DNA polymerases with higher pyrophosphorolysis...

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PMID: 11084870
DOI: 10.2144/00295rr03

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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