Article
Characterization of the glutamyl endopeptidase from Staphylococcus aureus expressed in Escherichia coli.
The FEBS journal - 1 Feb 2008
Nemoto Takayuki K, Ohara-Nemoto Yuko, Ono Toshio, Kobayakawa Takeshi, Shimoyama Yu, Kimura Shigenobu, Takagi Takashi
Abstract excerpt
V8 protease, a member of the glutamyl endopeptidase I family, of Staphylococcus aureus V8 strain (GluV8) is widely used for proteome analysis because of its unique substrate specificity and resistance to detergents. In this study, an Escherichia coli expression system for GluV8, as well as its homologue from Staphylococcus epidermidis (GluSE), was developed, and the roles of the prosegments and two specific amino...
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