Article
Rapid identification of somatic mutations in colorectal and breast cancer tissues using mismatch repair detection (MRD).
Human mutation - 1 Mar 2008
Bentivegna Steven, Zheng Jianbiao, Namsaraev Eugeni, Carlton Victoria E H, Pavlicek Adam, Moorhead Martin, Siddiqui Farooq, Wang Zhiyong, Lee Liana, Ireland James S, Suyenaga Kent, Willis Thomas D, Faham Malek, Seymour Albert B
Abstract excerpt
Mismatch repair detection (MRD) was used to screen 93 matched tumor-normal sample pairs and 22 cell lines for somatic mutations in 30 cancer relevant genes. Using a starting amount of only 150 ng of genomic DNA, we screened 102 kb of sequence for somatic mutations in colon and breast cancer. A total of 152 somatic mutations were discovered, encompassing previously reported mutations, such as BRAF V600E and KRAS...
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