Article
UmuD and RecA directly modulate the mutagenic potential of the Y family DNA polymerase DinB.
Molecular cell - 28 Dec 2007
Godoy Veronica G, Jarosz Daniel F, Simon Sharotka M, Abyzov Alexej, Ilyin Valentin, Walker Graham C
Abstract excerpt
DinB is the only translesion Y family DNA polymerase conserved among bacteria, archaea, and eukaryotes. DinB and its orthologs possess a specialized lesion bypass function but also display potentially deleterious -1 frameshift mutagenic phenotypes when overproduced. We show that the DNA damage-inducible proteins UmuD(2) and RecA act in concert to modulate this mutagenic activity. Structural modeling suggests that...
Topics
- Amino Acid Sequence
- Binding Sites
- Blotting, Far-Western
- DNA Polymerase beta
- DNA-Directed DNA Polymerase
- Escherichia coli
- Escherichia coli Proteins
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis