Targeted gene disruption of the endogenous c-abl locus by homologous recombination with DNA encoding a selectable fusion protein.

doi:10.1073/pnas.87.8.3210

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Article

Targeted gene disruption of the endogenous c-abl locus by homologous recombination with DNA encoding a selectable fusion protein.

Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 1990

Schwartzberg P L, Robertson E J, Goff S P

Abstract excerpt

We have introduced a substitution mutation into the c-abl locus of murine embryonic stem cells by homologous recombination between exogenously added DNA and the endogenous gene. Model constructs were initially generated that consisted of a promoterless selectable neomycin resistance marker inserted into the v-abl gene of the complete Abelson murine leukemia virus genome, designed to be expressed either as a...

Topics

Animals
Base Sequence
Cell Transformation, Neoplastic
Cells, Cultured
Cloning, Molecular
DNA
Immunoblotting
Mice
Molecular Sequence Data
Mutation
Plasmids

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein-Tyrosine Kinases

Proto-Oncogene Proteins

Proto-Oncogene Proteins c-abl

Proto-Oncogenes

Recombinant Fusion Proteins

Recombination, Genetic

Restriction Mapping

Source

Open in PubMed Open full text

Identifiers

PMID: 2183226
DOI: 10.1073/pnas.87.8.3210
PMCID: PMC53865

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2183226-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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