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Article

Targeted gene disruption of the endogenous c-abl locus by homologous recombination with DNA encoding a selectable fusion protein.

Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 1990

Schwartzberg P L, Robertson E J, Goff S P

Abstract excerpt

We have introduced a substitution mutation into the c-abl locus of murine embryonic stem cells by homologous recombination between exogenously added DNA and the endogenous gene. Model constructs were initially generated that consisted of a promoterless selectable neomycin resistance marker inserted into the v-abl gene of the complete Abelson murine leukemia virus genome, designed to be expressed either as a...

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Identifiers

PMID: 2183226
DOI: 10.1073/pnas.87.8.3210
PMCID: PMC53865

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