Measurement of translational accuracy in vivo: missense reporting using inactive enzyme mutants.

Abstract excerpt

The measurement and potential technological significance of in vivo missense errors are briefly reviewed. A recently developed approach is described in which reporter enzyme activity is generated by mistranslation of a gene coding for an inactive mutant form of the enzyme. Initial results obtained using the alpha subunit of E coli tryptophan synthetase and bacterial luciferase are discussed, as well as the...

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