Measurement of translational accuracy in vivo: missense reporting using inactive enzyme mutants.

Abstract excerpt

The measurement and potential technological significance of in vivo missense errors are briefly reviewed. A recently developed approach is described in which reporter enzyme activity is generated by mistranslation of a gene coding for an inactive mutant form of the enzyme. Initial results obtained using the alpha subunit of E coli tryptophan synthetase and bacterial luciferase are discussed, as well as the...

Topics

• Escherichia coli
• Luciferases
• Mutation
• Protein Biosynthesis
• Streptomycin
• Tryptophan Synthase