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Article

Enzymatic properties of mutant Escherichia coli tryptophan synthase alpha-subunits.

The Journal of biological chemistry - 25 Oct 1991

Lim W K, Sarkar S K, Hardman J K

Abstract excerpt

Thirty-nine mutant tryptophan synthase alpha subunits have been purified and analyzed (in the presence of the beta 2-subunit) for their enzymatic (kcat, Km) behavior in the reactions catalyzed by the alpha 2.beta 2 complex, the fully constituted form of this enzyme. The mutant alpha subunits, obtained by in vitro random, saturation mutagenesis of the encoding trpA gene, contain single amino acid substitutions at...

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PMID: 1939081

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