Article
[Detection of JAK2V617F mutation in patients with myeloproliferative disorders with TaqMan-MGB probe].
Zhonghua yi xue za zhi - 11 Sept 2007
Ruan Guo-rui, Chen Shan-shan, Li Ling-di, Liu Yan-rong, Qin Ya-zhen, Li Jin-lan, Ma Xi, Wang Feng-rong, Jiang Qian, Jiang Bin, Liu Kai-yan, Huang Xiao-jun
Abstract excerpt
OBJECTIVE: To develop a novel platform for detection of the JAK2V617F mutation in patients with myeloproliferative disorders (MPD) by real-time quantitative PCR. METHODS: TaqMan-MGB probe was constructed. Peripheral blood samples were collected from 374 MPD patients, 76 with polycythemia vera (PV), 38 with chronic myelogenous leukemia (CML), and 115 with essential thrombocythemia (ET), and 19 with idiopathic...
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