Article
[The investigation of JAK2 V617F point mutation in myeloproliferative disorders by allele-specific polymerase chain reaction in combination with sequence analysis].
Zhonghua yi xue za zhi - 14 Aug 2007
Zhang Su-jiang, Li Wei-da, Song Jun-hong, Xu Wei, Qiu Hong-xia, Li Jian-yong
Abstract excerpt
OBJECTIVE: To study the JAK2 V617F point mutation in myeloproliferative disorders (MPD) and explore the clinical significance. METHODS: We used Allele-specific polymerase chain reaction (AS-PCR) in combination with sequence analysis to detect the mutation in genomic DNA of peripheral blood mononuclear cells from 20 chronic myelogenous leukemia (CML) patients, 23 polycythaemia vera (PV), 40 essential...
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