Article
A xenobiotic-free culture system for human limbal epithelial stem cells.
Regenerative medicine - 1 Nov 2007
Notara Maria, Haddow David B, MacNeil Sheila, Daniels Julie T
Abstract excerpt
AIMS: Murine 3T3 feeder cells are commonly used for stem cell expansion. Although 'feeder-free' systems are being developed for a variety of stem cells including embryonic, the use of feeder cells currently remains optimal for the expansion of epithelial stem cells. In this study, MRC-5, a human embryonic fibroblast cell line, has been investigated for its potential use as a feeder layer in human limbal...
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