Article
Human amniotic epithelial cells as novel feeder layers for promoting ex vivo expansion of limbal epithelial progenitor cells.
Stem cells (Dayton, Ohio) - 1 Aug 2007
Chen Ying-Ting, Li Wei, Hayashida Yasutaka, He Hua, Chen Szu-Yu, Tseng David Y, Kheirkhah Ahmad, Tseng Scheffer C G
Abstract excerpt
Human amniotic epithelial cells (HAECs) are a unique embryonic cell source that potentially can be used as feeder layers for expanding different types of stem cells. In vivo, HAECs uniformly expressed pan-cytokeratins (pan-CK) and heterogeneously expressed vimentin (Vim). The two phenotypes expressing either pan-CK(+)/Vim(+) or pan-CK(+)/Vim(-) were maintained in serum-free media with high calcium. In contrast,...
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