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Article

Robust, persistent transgene expression in human embryonic stem cells is achieved with AAVS1-targeted integration.

Stem cells (Dayton, Ohio) - 1 Feb 2008

Smith Joseph R, Maguire Sean, Davis Lesley A, Alexander Morgan, Yang Fentang, Chandran Siddharthan, ffrench-Constant Charles, Pedersen Roger A

Abstract excerpt

Silencing and variegated transgene expression are poorly understood problems that can interfere with gene function studies in human embryonic stem cells (hESCs). We show that transgene expression (enhanced green fluorescent protein [EGFP]) from random integration sites in hESCs is affected by variegation and silencing, with only half of hESCs expressing the transgene, which is gradually lost after withdrawal of...

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PMID: 18024421
DOI: 10.1634/stemcells.2007-0039

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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