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Article

A semi-rational design strategy of directed evolution combined with chemical synthesis of DNA sequences.

Biological chemistry - 1 Dec 2007

Xiong Ai-Sheng, Peng Ri-He, Zhuang Jing, Liu Jin-Ge, Gao Feng, Xu Fang, Cai Bin, Yao Quan-Hong

Abstract excerpt

Directed evolution in vitro is a powerful molecular tool for the creation of new biological phenotypes. It is unclear whether it is more efficient to mutate an enzyme randomly or to mutate just the active sites or key sites. In this study, the strategy of a semi-rational design of directed evolut...

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Identifiers

PMID: 18020945
DOI: 10.1515/BC.2007.153

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-18020945-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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