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Article

Directed evolution of a beta-galactosidase from Pyrococcus woesei resulting in increased thermostable beta-glucuronidase activity.

Applied microbiology and biotechnology - 1 Dec 2007

Xiong Ai-Sheng, Peng Ri-He, Zhuang Jing, Li Xian, Xue Yong, Liu Jin-Ge, Gao Feng, Cai Bin, Chen Jian-Min, Yao Quan-Hong

Abstract excerpt

We performed directed evolution on a chemically synthesized 1,533-bp recombinant beta-galactosidase gene from Pyrococcus woesei. More than 200,000 variant colonies in each round of directed evolution were screened using the pYPX251 vector and host strain Rosetta-Blue (DE3). One shifted beta-galactosidase to beta-glucuronidase mutant, named YG6762, was obtained after four rounds of directed evolution and...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 17876575
DOI: 10.1007/s00253-007-1182-7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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