Article
Mutation of the arginine finger in the active site of Escherichia coli DbpA abolishes ATPase and helicase activity and confers a dominant slow growth phenotype.
Nucleic acids research - 1 Jan 2008
Elles Lisa M Sharpe, Uhlenbeck Olke C
Abstract excerpt
Escherichia coli DEAD-box protein A (DbpA) is an ATP-dependent RNA helicase with specificity for 23S ribosomal RNA. Although DbpA has been extensively characterized biochemically, its biological function remains unknown. Previous work has shown that a DbpA deletion strain is viable with little or no effect on growth rate. In attempt to elucidate a phenotype for DbpA, point mutations were made at eleven conserved...
Topics
- Adenosine Triphosphatases
- Amino Acid Motifs
- Arginine
- Binding Sites
- DEAD-box RNA Helicases
- Escherichia coli
- Escherichia coli Proteins
- Phenotype
- Point Mutation
- RNA