deaD, a new Escherichia coli gene encoding a presumed ATP-dependent RNA helicase, can suppress a mutation in rpsB, the gene encoding ribosomal protein S2.

Abstract excerpt

We have cloned and sequenced a new gene from Escherichia coli which encodes a 64-kDa protein. The inferred amino acid sequence of the protein shows remarkable similarity to eIF4A, a murine translation initiation factor that has an ATP-dependent RNA helicase activity and is a founding member of the D-E-A-D family of proteins (characterized by a conserved Asp-Glu-Ala-Asp motif). Our new gene, called deaD, was...

Topics

• Amino Acid Sequence
• Base Sequence
• Chromosome Mapping
• Cloning, Molecular
• Escherichia coli
• Genes, Bacterial
• Molecular Sequence Data
• Mutation
• RNA Helicases
• RNA Nucleotidyltransferases
• Ribosomal Proteins