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Article

A dominant negative mutant of the E. coli RNA helicase DbpA blocks assembly of the 50S ribosomal subunit.

Nucleic acids research - 1 Oct 2009

Sharpe Elles Lisa M, Sykes Michael T, Williamson James R, Uhlenbeck Olke C

Abstract excerpt

Escherichia coli DbpA is an ATP-dependent RNA helicase with specificity for hairpin 92 of 23S ribosomal RNA, an important part of the peptidyl transferase center. The R331A active site mutant of DbpA confers a dominant slow growth and cold sensitive phenotype when overexpressed in E. coli containing endogenous DbpA. Ribosome profiles from cells overexpressing DbpA R331A display increased levels of 50S and 30S...

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Identifiers

PMID: 19734347
DOI: 10.1093/nar/gkp711
PMCID: PMC2770675

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