Article
A Multipurpose receptor composed of promiscuous proteins. Analyte detection through pattern recognition.
Bioconjugate chemistry - 1 Jan 2000
Viljanen Johan, Larsson Jenny, Larsson Andréas, Broo Kerstin S
Abstract excerpt
A multipurpose receptor akin to the "electronic nose" was composed of coumarin-labeled mutants of human glutathione transferase A1. We have previously constructed a kit for site-specific modification of a lysine residue (A216K) using a thiol ester of glutathione (GSC-Cou bio) as a modifying reagent. In the present investigation, we scrambled the hydrophobic binding site (H-site) of the protein scaffold through...
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