Article
Mutation of immunoglobulin J chi splice sites does not affect the rearrangement frequency of J chi segments.
Research in immunology - 1 Jan 2000
Kallenbach S, Goodhardt M, Babinet C, Rougeon F
Abstract excerpt
The rabbit b9 kappa 1 locus contains 5 joining (J chi) gene segments, only two of which, J chi 1 and J chi 2, are utilized in assembly of a complete variable region gene (as shown by Akimenko, M.-A., Mariamé, B. and Rougeon, F. (1986) Proc. natl. Acad. Sci. (Wash.), 83, 5180-5183). J chi 4 and J chi 5 do not rearrange because of deficient recombination signal sequences. The J chi 3 gene segment is also not...
Topics
- Animals
- Base Sequence
- Blotting, Southern
- Gene Rearrangement, B-Lymphocyte
- Immunoglobulin Joining Region
- Immunoglobulin kappa-Chains
- Mice
- Mice, Transgenic
- Molecular Sequence Data
- Mutagenesis, Site-Directed