Article
Distribution and characterization of over 1000 T-DNA tags in rice genome.
The Plant journal : for cell and molecular biology - 1 Oct 2003
Chen Shuangyan, Jin Weizheng, Wang Mingyi, Zhang Fan, Zhou Jie, Jia Qiaojun, Wu Yunrong, Liu Feiyan, Wu Ping
Abstract excerpt
We generated T-DNA insertions throughout the rice genome for saturation mutagenesis. More than 1,000 flanking sequences were mapped on 12 rice chromosomes. Our results showed that T-DNA tags were not randomly spread on rice chromosomes and were preferentially inserted in gene-rich regions. Few insertions (2.4%) were found in repetitive regions. T-DNA insertions in genic (58.1%) and intergenic regions (41.9%)...
Topics
- DNA, Bacterial
- Gene Dosage
- Genome, Plant
- Internet
- Mutagenesis, Insertional
- Mutation
- Oryza
- Physical Chromosome Mapping