Article
Screening of genes related to methylglyoxal susceptibility.
Journal of microbiology (Seoul, Korea) - 1 Aug 2007
Kim Insook, Kim Joonho, Min Bumchan, Lee Changhan, Park Chankyu
Abstract excerpt
Methylglyoxal (MG) is a reactive metabolite known to accumulate in certain physiological conditions. We attempted to isolate genes associated with this metabolite by genome-wide mutagenesis with TnphoA derivative. After screening on methylglyoxal-containing plate, we obtained insertions in three different genes, ydbD, yjjQ, and yqiI, which gave rise to reproducible MG-sensitive phenotypes in glyoxalase-deficient...
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